Article
Development of gene-targeted SNP markers for genomic mapping in Pacific abalone Haliotis discus hannai Ino.
Molecular biology reports - 1 Dec 2010
Qi Haigang, Liu Xiao, Wu Fucun, Zhang Guofan
Abstract excerpt
Useful and novel DNA markers are needed for aquaculture genetics and breeding. In this study, we report the discovery and development of gene-targeted single nucleotide polymorphisms (SNPs) for genomic mapping in the Pacific abalone Haliotis discus hannai Ino. Single EST or EST-contigs from 66 genes that had positive BLASTx matches (E-value ≤ 1e-8) were used for polymerase chain reaction (PCR) amplification. PCR...
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