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Article

A simple and efficient method for extraction of PCR-amplifiable DNA from chicken eggshells.

Animal science journal = Nihon chikusan Gakkaiho - 1 Apr 2009

Rikimaru Kazuhiro, Takahashi Hideaki

Abstract excerpt

Recently, we reported a method for discriminating a Japanese brand of chicken, the Hinai-jidori. As an application of this method for discriminating Hinai-jidori eggs, we here report an efficient method for extracting maternal DNA from eggshells. Eggshell powder was completely decalcified with EDTA solution, and then DNA was isolated by conventional phenol-chloroform extraction and ethanol precipitation. The...

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Identifiers

PMID: 20163594
DOI: 10.1111/j.1740-0929.2008.00624.x

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