Article
De novo generation of cymbidium ringspot virus defective interfering RNA.
The Journal of general virology - 1 Mar 1991
Burgyan J, Rubino L, Russo M
Abstract excerpt
Nicotiana clevelandii plants were inoculated with cymbidium ringspot tombusvirus RNA synthesized in vitro, after which further passages were made by sap inoculation. During the third passage, low Mr RNA species appeared which had the characteristics of deletion mutants of genomic RNA. Sequence analysis of several of these defective interfering RNAs suggested a possible evolution of smaller from larger molecules....
Topics
- Base Sequence
- Blotting, Northern
- Cloning, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Plant Viruses
- Plants, Toxic
- RNA, Viral
- Recombination, Genetic
- Nicotiana