Article
[Optimization of the REP-PCR molecular classification method of Salmonella and its application in drug-resistant strains].
Wei sheng yan jiu = Journal of hygiene research - 1 Nov 2009
Wang Chuan, Yu Qian, Ma Liya, Zhang Chaowu, Pei Xiaofang, Liu Hengchuan
Abstract excerpt
OBJECTIVE: To optimize the reaction conditions of REP-PCR molecular classification method of Salmonella; primarily apply it in drug-resistant strains, and supply data to a system of Salmonella homology tracing. METHODS: Genomic DNA of Salmonella enteritidis 510041 was used as the template for PCR. Target sequences in 510041 genomic DNA were amplified with the primers designed according to the references. To...
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