Article
Repetitive extragenic palindromic PCR for study of Streptococcus mutans diversity and transmission in human populations.
Journal of clinical microbiology - 1 Feb 2010
Moser S A, Mitchell S C, Ruby J D, Momeni S, Osgood R C, Whiddon J, Childers N K
Abstract excerpt
Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular epidemiological study. Repetitive extragenic palindromic PCR (rep-PCR) is less time-consuming and more suitable for analyzing large numbers of bacterial strains in human populations. PFGE and rep-PCR provide comparable genotyping results for investigating Streptococcus mutans diversity and transmission.
Topics
- Bacterial Typing Techniques
- Child
- Child, Preschool
- Cluster Analysis
- DNA Fingerprinting
- Electrophoresis, Gel, Pulsed-Field
- Female
- Genotype
- Humans
- Infant
- Male
