Article
Serotyping pneumococcal meningitis cases in the African meningitis belt by use of multiplex PCR with cerebrospinal fluid.
Journal of clinical microbiology - 1 Feb 2010
Njanpop Lafourcade Berthe-Marie, Sanou Oumarou, van der Linden Mark, Levina Natalia, Karanfil Meryem, Yaro Seydou, Tamekloe Tsidi A, Mueller Judith E
Abstract excerpt
We reformulated a multiplex PCR algorithm for serotyping of pneumococcal meningitis directly on cerebrospinal fluid (CSF). Compared to established methods on isolates, CSF-based PCR had at least 80% sensitivity and 100% specificity. In regional meningitis surveillance, CSF-based PCR increased the...
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