Article
Identification of stress-related proteins in Escherichia coli using the pollutant cis-dichloroethylene.
Journal of applied microbiology - 1 Jun 2010
Lee J, Hiibel S R, Reardon K F, Wood T K
Abstract excerpt
AIMS: To complement our proteome study, whole-transcriptome analyses were utilized here to identify proteins related to degrading cis-1,2-dichloroethylene (cis-DCE). METHODS AND RESULTS: Metabolically engineered Escherichia coli strains were utilized expressing an evolved toluene ortho-monooxygenase along with either (i) glutathione S-transferase and altered gamma-glutamylcysteine synthetase or (ii) a rationally...
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