Article
Viral metagenomics: analysis of begomoviruses by illumina high-throughput sequencing.
Viruses - 12 Mar 2014
Idris Ali, Al-Saleh Mohammed, Piatek Marek J, Al-Shahwan Ibrahim, Ali Shahjahan, Brown Judith K
Abstract excerpt
Traditional DNA sequencing methods are inefficient, lack the ability to discern the least abundant viral sequences, and ineffective for determining the extent of variability in viral populations. Here, populations of single-stranded DNA plant begomoviral genomes and their associated beta- and alpha-satellite molecules (virus-satellite complexes) (genus, Begomovirus; family, Geminiviridae) were enriched from total...
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