Article
Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies.
Biological research - 1 Jan 2009
Marrero Patricia, Fregel Rosa, Cabrera Vicente M, Nogales Manuel
Abstract excerpt
DNA extraction methods for genotyping non-invasive samples have led to great advances in molecular research for ecological studies, and have been particularly useful for analyzing threatened species. However, scarce amounts of fragmented DNA and the presence of Taq polymerase inhibitors in non-invasive samples are potential problems for subsequent PCR amplifications. In this study we describe a novel technique...
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