Article
Rapid semiautomated subtyping of influenza virus species during the 2009 swine origin influenza A H1N1 virus epidemic in Milwaukee, Wisconsin.
Journal of clinical microbiology - 1 Sept 2009
Bose Michael E, Beck Eric T, Ledeboer Nate, Kehl Sue C, Jurgens Lisa A, Patitucci Teresa, Witt Lorraine, LaGue Elizabeth, Darga Patrick, He Jie, Fan Jiang, Kumar Swati, Henrickson Kelly J
Abstract excerpt
In the spring of 2009, a novel influenza A (H1N1) virus (swine origin influenza virus [S-OIV]) emerged and began causing a large outbreak of illness in Milwaukee, WI. Our group at the Midwest Respiratory Virus Program laboratory developed a semiautomated real-time multiplex reverse transcription-PCR assay (Seasonal), employing the NucliSENS easyMAG system (bioMérieux, Durham, NC) and a Raider thermocycler...
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