Article
Direct sequencing of Legionella pneumophila from respiratory samples for sequence-based typing analysis.
Journal of clinical microbiology - 1 Sept 2009
Coscollá Mireia, González-Candelas Fernando
Abstract excerpt
We have developed a procedure to test the efficiency and reliability of sequencing of Legionella pneumophila genes directly from respiratory samples and have compared the results with those derived from cultured isolates. We tried to obtain the nucleotide sequences of six protein-coding loci included in the sequence-based typing scheme for Legionella pneumophila and three intergenic regions from 132 samples...
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