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Article

Degradation of 2-methylaniline in Rhodococcus rhodochrous: cloning and expression of two clustered catechol 2,3-dioxygenase genes from strain CTM.

Journal of general microbiology - 1 Aug 1991

Schreiner A, Fuchs K, Lottspeich F, Poth H, Lingens F

Abstract excerpt

Rhodococcus rhodochrous strain CTM degrades 2-methylaniline mainly via the meta-cleavage pathway. Conversion of the metabolite 3-methylcatechol was catalysed by an Mr 156,000 catechol 2,3-dioxygenase (C23OI) comprising four identical subunits of Mr 39,000. The corresponding gene was detected by using an oligonucleotide as a gene probe. This oligonucleotide was synthesized on the basis of a partial amino acid...

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PMID: 1955878
DOI: 10.1099/00221287-137-8-2041

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