Article
Evaluation of two ELISA assay kits against RT-PCR for diagnosis of dengue virus infection in a hospital setting in Karachi, Pakistan.
JPMA. The Journal of the Pakistan Medical Association - 1 Jun 2009
Khan Erum, Mehraj Vikram, Nasir Amna, Khan Nabil Ahmad, Billoo Bushra, Moatter Tariq, Hasan Rumina
Abstract excerpt
OBJECTIVE: To evaluate two commercially available ELISA-based kits against RT-PCR for the diagnosis of dengue virus infection in a Tertiary Care center in Karachi. METHODS: During the 2006 Dengue outbreak, sera were collected from patients clinically classified as dengue fever and graded according to WHO grading. Out of these, 83 samples were selected randomly and analyzed using two different commercial kits...
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