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Detection of <i>Mycobacterium tuberculosis</i> in Sputum Samples Using a Polymerase Chain Reaction

1 Nov 1991

Abstract excerpt

A polymerase chain reaction (PCR) assay for the rapid detection of Mycobacterium tuberculosis in sputum samples is described. The target DNA is a 123-base pair (bp) segment of IS6110, which is repeated in the M. tuberculosis chromosome and is specific for the M. tuberculosis complex. Methodology used to lyse the mycobacteria, extract the DNA, and amplify the 123-bp target DNA is presented. The amplified PCR...

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PMID: 1952448
DOI: 10.1164/ajrccm/144.5.1160

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