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Molecular characterization of Irish E. coli O157:H7 isolates of human, bovine, ovine and porcine origin.

Journal of applied microbiology - 1 Oct 2009

Lenahan M, O'Brien S B, Byrne C, Ryan M, Kennedy C-A, McNamara E B, Fanning S, Sheridan J J, Sweeney T

Abstract excerpt

AIMS: To determine the degree of relatedness between isolates of Escherichia coli O157:H7 of human, bovine, ovine and porcine origin. METHODS AND RESULTS: Escherichia coli O157:H7 isolates were compared using (i) PFGE XbaI patterns, (ii) PCR profiles of virulence genes and (iii) the DNA sequences of genes reported to play a role in pathogenicity. The 77 E. coli O157:H7 isolates demonstrated 49 different PFGE...

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PMID: 19486385
DOI: 10.1111/j.1365-2672.2009.04320.x

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