Molecular characterization of Irish E. coli O157:H7 isolates of human, bovine, ovine and porcine origin.

doi:10.1111/j.1365-2672.2009.04320.x

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Article

Molecular characterization of Irish E. coli O157:H7 isolates of human, bovine, ovine and porcine origin.

Journal of applied microbiology - 1 Oct 2009

Lenahan M, O'Brien S B, Byrne C, Ryan M, Kennedy C-A, McNamara E B, Fanning S, Sheridan J J, Sweeney T

Abstract excerpt

AIMS: To determine the degree of relatedness between isolates of Escherichia coli O157:H7 of human, bovine, ovine and porcine origin. METHODS AND RESULTS: Escherichia coli O157:H7 isolates were compared using (i) PFGE XbaI patterns, (ii) PCR profiles of virulence genes and (iii) the DNA sequences of genes reported to play a role in pathogenicity. The 77 E. coli O157:H7 isolates demonstrated 49 different PFGE...

Topics

Animals
Cattle
DNA, Bacterial
Electrophoresis, Gel, Pulsed-Field
Escherichia coli Infections
Escherichia coli O157
Escherichia coli Proteins
Feces
Humans
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymorphism, Single Nucleotide

Sequence Alignment

Sequence Analysis, DNA

Sheep, Domestic

Swine

Virulence

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Open in PubMed

Identifiers

PMID: 19486385
DOI: 10.1111/j.1365-2672.2009.04320.x

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