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Article

Development of an assay for the detection and quantification of the measles virus nucleoprotein (N) gene using real-time reverse transcriptase PCR.

Journal of medical microbiology - 1 May 2009

Akiyama Miho, Kimura Hirokazu, Tsukagoshi Hiroyuki, Taira Katsuya, Mizuta Katsumi, Saitoh Mika, Nagano Manami, Sutoh Asuka, Noda Masahiro, Morita Yukio, Sakatsume Osamu, Okabe Nobuhiko, Tashiro Masato

Abstract excerpt

We developed a new quantification method for the measles virus (MeV) nucleoprotein (N) gene using real-time reverse transcriptase PCR. This method allowed us to quantify 10(1)-10(7) copies per reaction (corresponding to 5x10(-1)-5x10(5) copies microl(-1)) of the MeV N gene. We also quantified the MeV N gene from the throat swabs of 22 patients with measles as well as the MeV genotypes A, D3, D5, D9 and H1 in...

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Identifiers

PMID: 19369526
DOI: 10.1099/jmm.0.005439-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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