Article
Genotyping measles virus by real-time amplification refractory mutation system PCR represents a rapid approach for measles outbreak investigations.
Journal of clinical microbiology - 1 Feb 2006
Waku-Kouomou Diane, Alla Amal, Blanquier Bariza, Jeantet Damien, Caidi Hayat, Rguig Ahmed, Freymuth François, Wild Fabian T
Abstract excerpt
Real-time PCR has been developed to genotype measles virus (MV) isolates. MV strains circulating in epidemics in Gabon in 1984, Cameroon in 2001, Morocco in 2003, and France in 2004 were investigated. We developed a real-time amplification refractory mutation system PCR (RT-AMRS PCR) using SYBR green fluorescent dye. Six pairs of primers for RT-ARMS PCR were designed to specifically amplify genotypes A, B2, B3.1,...
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