In vitro reconstitution of recombinant lamin A and a lamin A mutant lacking the carboxy-terminal tail.

Article

In vitro reconstitution of recombinant lamin A and a lamin A mutant lacking the carboxy-terminal tail.

European journal of cell biology - 1 Aug 1991

Gieffers C, Krohne G

Abstract excerpt

Xenopus lamin A and a lamin A mutant lacking the complete 280 amino acid long carboxy-terminal tail were expressed in bacteria and purified from inclusion bodies. Electron microscopic analysis of lamin A dimers revealed that the carboxy-terminal 280 amino acids correspond to the globular domain seen in rotary-shadowed wild-type lamin and that the rodlike domain consists of the short non-helical amino terminus and...

Topics

Animals
Base Sequence
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Gene Expression
Genes, Bacterial
Lamin Type A
Lamins
Microscopy, Electron
Molecular Sequence Data
Mutation

Nuclear Proteins

Recombinant Proteins

Xenopus

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