Article
Identification of human esterase D subunits from the homodimeric and heterodimeric forms of five phenotypes by a new two-dimensional isoelectric focusing method.
Electrophoresis - 1 May 1991
Alonso A, Visedo G, Sancho M, Fernández-Piqueras J
Abstract excerpt
A new two-dimensional isoelectric focusing method was developed to identify the human esterase D (EsD) subunits from the homodimeric and heterodimeric forms of five EsD phenotypes. EsD polymorphism was also analyzed by one-dimensional isoelectric focusing under reducing and mild denaturing conditions to study the influence of dithiothreitol and low concentrations of urea on the focusing pattern of the EsD dimers.
Topics
- Alleles
- Carboxylesterase
- Carboxylic Ester Hydrolases
- Chromosomes, Human, Pair 13
- Electrophoresis, Polyacrylamide Gel
- Erythrocytes
- Genes
- Genetic Markers
- Humans
- Isoelectric Focusing