Article
An arginine to lysine substitution in the bZIP domain of an opaque-2 mutant in maize abolishes specific DNA binding.
Genes & development - 1 Feb 1991
Aukerman M J, Schmidt R J, Burr B, Burr F A
Abstract excerpt
The opaque-2 (o2) locus in maize encodes a transcription factor involved in the regulation of zein storage proteins. We have shown previously that the O2 protein contains a leucine zipper domain that binds to promoters of 22-kD zein genes. In this paper we characterize an EMS-induced o2 allele, o2-676, that causes a 50% reduction in zein. We have found that the o2-676 mutant protein does not show specific...
Topics
- Amino Acid Sequence
- Arginine
- Blotting, Northern
- DNA-Binding Proteins
- Electrophoresis, Gel, Two-Dimensional
- Leucine Zippers
- Lysine
- Molecular Sequence Data
- Mutation
- Phenotype
- Plant Proteins