Article
Mutations of the 22- and 27-kD zein promoters affect transactivation by the Opaque-2 protein.
The Plant cell - 1 Jun 1992
Ueda T, Waverczak W, Ward K, Sher N, Ketudat M, Schmidt R J, Messing J
Abstract excerpt
By utilizing a homologous transient expression system, we have demonstrated that the Opaque-2 (O2) gene product O2 confers positive trans-regulation on a 22-kD zein promoter. This trans-acting function of the O2 protein is mediated by its sequence-specific binding to a cis element (the O2 target site) present in the 22-kD zein promoter. A multimer of a 32-bp promoter fragment containing this O2 target site...
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