Article
Consensus RT-nested PCR detection of yellow head complex genotypes in penaeid shrimp.
Journal of virological methods - 1 Nov 2008
Wijegoonawardane Priyanjalie K M, Cowley Jeff A, Walker Peter J
Abstract excerpt
A consensus RT-nested (n)PCR is described that detects the six distinct genotypic variants in the yellow head virus (YHV) complex. The PCR primers targeted ORF1b gene regions more highly conserved amongst the reference strains of YHV (genotype 1) and gill-associated virus (GAV, genotype 2) and a set of 57 field isolates containing multiple representatives of each genotype. The test employed short PCR (359 bp) and...
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