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Article

Direct detection of dystrophin gene rearrangements by analysis of dystrophin mRNA in peripheral blood lymphocytes.

American journal of human genetics - 1 Aug 1991

Roberts R G, Barby T F, Manners E, Bobrow M, Bentley D R

Abstract excerpt

Using 10 overlapping nested sets of primers and using peripheral blood lymphocyte (PBL) total RNA as template, we have developed a system, based on PCR, which allows the rapid production of double-stranded cDNA corresponding to the entire coding sequence of the dystrophin gene. The product can be visualized on native minigels by ethidium staining and directly sequenced after gel purification. We have used this...

Topics

Base Sequence
Dystrophin
Frameshift Mutation
Gene Rearrangement
Genotype
Humans
Lymphocytes
Molecular Sequence Data
Muscular Dystrophies
Oligonucleotide Probes
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymerase Chain Reaction

RNA Splicing

RNA, Messenger

Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 1867192
PMCID: PMC1683309

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1867192-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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