Article
Simultaneous assessment of the effects of exonic mutations on RNA splicing and protein functions.
Biochemical and biophysical research communications - 5 Sept 2008
Ho Po-yi, Huang Miao-Zeng, Fwu Victor Tramjay, Lin Sheng-Chieh, Hsiao Kwang-Jen, Su Tsung-Sheng
Abstract excerpt
To simultaneously assess the effects of exonic mutations on RNA splicing and protein functions, we report here an intron-inclusive cDNA (Intinc) expression system. As a test model, twenty-four mutations in exon 9 of the phenylalanine hydroxylase (PAH) gene were examined in an Intinc expression plasmid composed of the PAH cDNA with the exon 9 flanked by its authentic introns. When the PAH enzyme activities from...
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