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Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis

29 Apr 2008

Abstract excerpt

BACKGROUND: The sensitivity and specificity of 18S rRNA polymerase chain reaction (PCR) in the detection of fungal aetiology of microbial keratitis was determined in thirty patients with clinical diagnosis of microbial keratitis. METHODS: Corneal scrapings from patients were used for Gram stain, culture and PCR analysis. PCR was performed with primer pairs targeted to the 18S rRNA gene. The result of the PCR was...

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PMID: 18445283
DOI: 10.1186/1471-2415-8-7

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