Article
Development of single‐tube real‐time PCR assay for the rapid detection of Aspergillus and Fusarium—The two most common causative agents in fungal keratitis
25 Jun 2023
Abstract excerpt
BACKGROUND: To compare the performance of conventional, semi-nested and real-time panfungal ITS PCRs for diagnosing fungal keratitis (FK) and develop genus-specific real-time PCR for the most common aetiology of FK. METHODS: This multicentric study includes 232 corneal samples from suspected FK patients from four centres across India between November 2019 through August 2021. A total of 87 corneal buttons were...
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