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Quantitative Polymerase Chain Reaction Assay for Largemouth Bass Virus

1 Dec 2007

Abstract excerpt

The use of quantitative polymerase chain reaction (QPCR) to test for largemouth bass virus (LMBV) was evaluated during a challenge experiment in which largemouth bass Micropterus salmoides were immersed in the type strain of LMBV. The real-time PCR and cell culture methods were both used to measure LMBV present in the inoculum. Additional samples tested by QPCR included gill, gonad, kidney, liver, mucus, spleen,...

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Identifiers

PMID: 18333479
DOI: 10.1577/h07-009.1

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