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Article

Nuclear staining identifies two populations of human sperm with different DNA fragmentation extent and relationship with semen parameters

8 Mar 2008

Abstract excerpt

BACKGROUND: Sperm DNA fragmentation is a possible predictive parameter for male fertility status. The occurrence of M540 bodies in semen of subfertile subjects affects flow cytometric investigations in sperm. We set up a new method to evaluate DNA fragmentation excluding M540 bodies. METHODS: DNA fragmentation was evaluated by flow cytometry in semen of 75 subjects both by terminal deoxynucleotidyl...

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PMID: 18326515
DOI: 10.1093/humrep/den058

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