Article
Molecular variability of Hop stunt viroid: identification of a unique variant with a tandem 15-nucleotide repeat from naturally infected plum tree.
Biochemical genetics - 1 Apr 2008
Yang Yuan-Ai, Wang Hong-Qing, Wu Zu-Jian, Cheng Zhuo-Min, Li Shi-Fang
Abstract excerpt
For this study, 68 plum samples were collected from 12 provinces of China. Low molecular weight RNAs were extracted and used for dot-blot, reverse transcription-polymerase chain reaction (RT-PCR), return-polyacrylamide gel electrophoresis, and biological indexing using cucumber. Results showed that 15 out of the 68 plum samples were positive for Hop stunt viroid (HSVd). Four positive samples were selected for...
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