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A multiplex polymerase chain reaction assay for the identification of Mannheimia haemolytica, Mannheimia glucosida and Mannheimia ruminalis.

Veterinary microbiology - 27 Jul 2008

Alexander Trevor W, Cook Shaun R, Yanke L Jay, Booker Calvin W, Morley Paul S, Read Ron R, Gow Sheryl P, McAllister Tim A

Abstract excerpt

The objective of this study was to design a multiplex PCR assay to identify Mannheimia haemolytica, Mannheimia glucosida and Mannheimia ruminalis. The multiplex PCR included primer sets HP, amplifying a DNA region from an unknown hypothetical protein, Lkt and Lkt2, amplifying different regions of the leukotoxinD gene, and 16S to amplify universal bacterial sequences of the 16S rRNA gene. Based on positive...

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PMID: 18308486
DOI: 10.1016/j.vetmic.2008.01.001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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