Article
[Genotyping 238 HBV strains using type-specific primer PCR combined with type-specific nucleotide analysis].
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology - 1 Feb 2008
Zeng Ai-Zhong, Huang Ai-Long, Guo Jin-Jun, Deng Xiao-Yan, Li Qing-Ling, Huang Wen-Xiang
Abstract excerpt
OBJECTIVE: To establish a set of suitable and reliable methods for HBV genotyping and to study the distribution of HBV genotypes. METHODS: Type-specific nucleotides were searched through alignment of S genes (more than 1000 sequences) listed in GenBank. Then, type-specific primers were designed and type-specific primer PCR was used to genotype the 238 HBV strains. S genes of the untyped strains were further...
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