Article
A metagenomic analysis of soil bacteria extends the diversity of quorum-quenching lactonases.
Environmental microbiology - 1 Mar 2008
Riaz Kashif, Elmerich Claudine, Moreira David, Raffoux Aurélie, Dessaux Yves, Faure Denis
Abstract excerpt
A metagenomic library of 10,121 clones, generated from bacteria inhabiting a pasture soil from France, was screened for the presence of fosmids conferring either N-acylhomoserine lactone (NAHL) synthesis or NAHL degradation ability upon their Escherichia coli host. No clone producing NAHLs was identified whereas one, containing a 31 972 bp insert in fosmid p2H8, allowed NAHL degradation. This led to the cloning...
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