Article
Identification of deletion and duplication genotypes of the PMP22 gene using PCR-RFLP, competitive multiplex PCR, and multiplex ligation-dependent probe amplification: a comparison.
Electrophoresis - 1 Feb 2008
Hung Chia-Cheng, Lee Chien-Nan, Lin Chia-Yun, Cheng Wen-Fang, Chen Chi-An, Hsieh Sung-Tsang, Yang Chih-Chao, Jong Yuh-Jyh, Su Yi-Ning, Lin Win-Li
Abstract excerpt
We evaluated the efficacy of PCR-RFLP, competitive multiplex PCR, and a commercially available system of multiplex ligation-dependent probe amplification (MLPA) for the determination of deletion and duplication genotypes of the PMP22 gene. We compared the methods for efficiency, sensitivity, and specificity. We determined the gene dosage of the PMP22 gene via PCR-RFLP, competitive multiplex PCR, and MLPA. To...
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