Article
Deletion analysis of the catalase-encoding gene (catB) promoter from Aspergillus oryzae.
Bioscience, biotechnology, and biochemistry - 1 Jan 2008
Hisada Hiromoto, Sano Motoaki, Hata Yoji, Abe Yasuhisa, Machida Masayuki
Abstract excerpt
The catalase-encoding gene (catB) is expressed strongly in Aspergillus oryzae. To identify the transcription regulatory elements involved in strong expression, we did promoter deletion analysis using beta-glucuronidase (GUS) as a reporter and an electrophoretic gel mobility shift assay (EMSA) systematically. The deletion 200-bp sequence from -1,000 to -800 in the 1,400-bp catB promoter caused a drastic decrease...
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