Article
[Establishment of fluorescent amplified fragment length polymorphism in Vibrio cholerae and evaluation in molecular typing].
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi - 1 Jun 2007
Lou Jing, Diao Bao-wei, Wang Hong-xia, Cui Zhi-gang, Qi Guo-ming, Kan Biao
Abstract excerpt
OBJECTIVE: To develop fluorescent amplified fragment length polymorphism (AFLP) method and to evaluate the its typing capability with pulsed-field gel electrophoresis (PFGE) in molecular typing of Vibrio cholerae. METHODS: Forty-seven strains of V. cholerae, with different PFGE patterns, were selected as the reference group to optimize the selective primers of AFLP analysis. Eighty-three strains including 20...
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