Fluorescent amplified-fragment length polymorphism genotyping of Salmonella enterica subsp. enterica serovars and comparison with pulsed-field gel electrophoresis typing.

Abstract excerpt

We have performed the fluorescently labeled amplified-fragment length polymorphism (FAFLP) method on 97 strains of the food-borne pathogen Salmonella enterica subsp. enterica comprising seven different serovars using the restriction enzymes EcoRI and MseI. From the total FAFLP fingerprinted strains, 81 were compared with pulsed-field gel electrophoresis (PFGE) typing of the same strains. The FAFLP method showed a...

Topics

• Bacterial Typing Techniques
• Electrophoresis, Gel, Pulsed-Field
• Fluorescence
• Genotype
• Humans
• Polymorphism, Restriction Fragment Length
• Salmonella Food Poisoning
• Salmonella enterica