Article
A simple multiplex PCR assay for diagnosing virulent Helicobacter pylori infection in human gastric biopsy specimens from subjects with gastric carcinoma and other gastro-duodenal diseases.
Journal of applied microbiology - 1 Dec 2007
Tiwari S K, Khan A A, Manoj G, Ahmed S, Abid Z, Habeeb A, Habibullah C M
Abstract excerpt
AIM: To evaluate and develop a multiplex polymerase chain reaction (PCR) assay for diagnosing and specific identification of virulent Helicobacter pylori strains and their main virulence genes cagA, cagE, cagT, vacA and hrgA. METHODS AND RESULTS: Genomic DNA from 82 gastric tissues was screened. A master pool of all the ingredients of multiplex reaction was prepared for amplification. Amplicons were sequenced to...
Topics
- Adult
- Biopsy
- Chi-Square Distribution
- DNA Primers
- DNA, Bacterial
- Female
- Gastric Mucosa
- Gastritis
- Gastrointestinal Diseases
- Genotype
- Helicobacter Infections
- Helicobacter pylori
- Humans