Article
In Vitro Decondensation of Nuclear Chromatin of Human Spermatozoa: Assessing Fertilizing Potential
1 Jan 1991
Abstract excerpt
Spermatozoan nuclear chromatin is in a highly condensed state prior to fertilization. In vivo decondensation occurs in the ooplasm and is essential for successful fertilization and the formation of male pronucleus and the zygote to occur. The chromatin of spermatozoa and nucleus can undergo in vitro decondensation with sodium dodecyl sulfate (SDS) and 6 mM ethylene diamine tetraacetic acid (EDTA). The ability of...
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