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Article

Limitations of Green Fluorescent Protein as a Cell Lineage Marker

5 Jul 2007

Abstract excerpt

The enhanced green fluorescent protein (GFP) reporter has been widely adopted for tracking cell lineage. Here, we compare three transgenic mouse strains in which GFP is considered "ubiquitously expressed," with the GFP transgene under control of the chicken beta-actin (CBA) or human ubiquitin C (UBC) promoter. We compared the expression of GFP using flow cytometry, direct tissue fluorescence, and immunostaining...

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Identifiers

PMID: 17615263
DOI: 10.1634/stemcells.2007-0241

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