Article
A statistical pattern recognition approach for determining cellular viability and lineage phenotype in cultured cells and murine bone marrow.
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Aug 2007
Quinn John, Fisher Paul W, Capocasale Renold J, Achuthanandam Ram, Kam Moshe, Bugelski Peter J, Hrebien Leonid
Abstract excerpt
BACKGROUND: Cellular binding of annexin V and membrane permeability to 7-aminoactinomycin D (7AAD) are important tools for studying apoptosis and cell death by flow cytometry. Combining viability markers with cell surface marker expression is routinely used to study various cell lineages. Current classification methods using strict thresholds, or "gates," on the fluorescent intensity of these markers are...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.