Article
[Isolation and culturation, phenotype detection of rat bone marrow mesenchymal stem cells].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 May 2007
Wang Chao, Xu Yun, Song Wen-gang, Chang Wei-shan
Abstract excerpt
AIM: To investigate the more effective methods of isolation, culture of mesenchymal stem cell (MSC), and examine the surface phenotype of MSC. MSC were separated from rats bone marrow by plastic adherence methods and purified by controlling the time of digestion combined with adhesion separation, and proliferated in culture flasks that were coated with I collogen. METHODS: The morphology of MSC were studied with...
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