Article
[Isolating and culturing rat marrow mesenchymal stem cells and studying their phenotypical and functional properties].
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition - 1 Oct 2003
Zhang Bensi, Wang Fan, Deng Li, Dun Aishe, Dong Lihua, Li Jian, Yang Huijun
Abstract excerpt
OBJECTIVE: To establish a method for isolating and cultivating mesenchymal stem cells (MSCs) from SD rat bone marrow and to study their phenotypical and functional properties. METHODS: MSCs from rat bone marrow were separated and purified by gradient centrifugation and adherence to the culture plastic; then the cells were expanded by subculture successively. The growth curves were drawn, and the morphology was...
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