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Article

PCR amplification of hydrogen cyanide biosynthetic locus hcnAB in Pseudomonas spp.

Journal of microbiological methods - 1 Jul 2007

Svercel M, Duffy B, Défago G

Abstract excerpt

A PCR-based assay targeting hcnAB, essential genes for hydrogen cyanide (HCN) biosynthesis, allowed sensitive detection of HCN(+) pseudomonads between logs 2.9 and 3.5 cells per PCR reaction tube. RFLP analysis revealed 13 allele combinations among selected 2,4-diacetylphloroglucinol-producing (Phl(+))HCN(+), and 13 alleles in Phl(-) HCN(+) strains from a global collection.

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Identifiers

PMID: 17475352
DOI: 10.1016/j.mimet.2007.03.018

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