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Real-time RT-PCR for H5N1 avian influenza A virus detection

19 Apr 2007

Abstract excerpt

The recent recurrence of highly pathogenic avian influenza virus A H5N1 was firstly reported in mid-December 2003 and continued through 2005. This study describes a sensitive and specific real-time RT-PCR method for the detection of influenza A subtype H5 and for monitoring virus loads. Using serial dilutions of influenza A H5N1 cultures, this assay reproducibly determined the lowest detection limit to be...

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PMID: 17446281
DOI: 10.1099/jmm.0.47014-0

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