Article
A specific polymerase chain reaction based on the gyrB gene sequence and subsequent restriction fragment length polymorphism analysis of Pasteurella pneumotropica isolates from laboratory mice.
Journal of the American Association for Laboratory Animal Science : JAALAS - 1 Mar 2007
Hayashimoto Nobuhito, Ueno Masami, Takakura Akira, Itoh Toshio
Abstract excerpt
For a molecular identification and typing tool, we developed a specific polymerase chain reaction (PCR) based on the gyrB gene sequence and a subsequent restriction fragment length polymorphism (RFLP) analysis using the products amplified from the specific PCR to facilitate discrimination of biotypes of Pasteurella pneumotropica from laboratory mice. Appropriate PCR products, a 1039-basepair fragment for biotype...
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