Article
Development of quantitative polymerase chain reaction assays for allelic discrimination of gangliosidoses in cats.
American journal of veterinary research - 1 Mar 2007
Wang Chi-Young J, Smith Bruce F
Abstract excerpt
OBJECTIVE: To develop quantitative PCR (qPCR) assays with allele-specific primers to provide a rapid and accurate diagnostic and screening test for the 3 mutations identified as causes of gangliosidoses in domestic cats. SAMPLE POPULATION: DNA samples obtained from archived feline blood samples submitted for GM1 and GM2 testing. PROCEDURES: A qPCR assay was developed for each mutation to monitor the efficiency of...
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