Article
Enzymatic formation of unnatural cytokinin analogs by adenylate isopentenyltransferase from mulberry.
Biochemical and biophysical research communications - 13 Apr 2007
Abe Ikuro, Tanaka Hideya, Abe Tsuyoshi, Noguchi Hiroshi
Abstract excerpt
A cDNA encoding adenylate isopentenyltransferase (AIPT) was cloned from young leaves of mulberry (Morus alba) by a homology-based RT-PCR. A recombinant enzyme expressed in Escherichia coli catalyzed prenyl transfer from DMAPP to the N6 amino group of ADP and ATP, respectively, while AMP was a poo...
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