Article
Arabidopsis vacuolar sorting mutants (green fluorescent seed) can be identified efficiently by secretion of vacuole-targeted green fluorescent protein in their seeds.
The Plant cell - 1 Feb 2007
Fuji Kentaro, Shimada Tomoo, Takahashi Hideyuki, Tamura Kentaro, Koumoto Yasuko, Utsumi Shigeru, Nishizawa Keito, Maruyama Nobuyuki, Hara-Nishimura Ikuko
Abstract excerpt
Two Arabidopsis thaliana genes have been shown to function in vacuolar sorting of seed storage proteins: a vacuolar sorting receptor, VSR1/ATELP1, and a retromer component, MAIGO1 (MAG1)/VPS29. Here, we show an efficient and simple method for isolating vacuolar sorting mutants of Arabidopsis. The method was based on two findings in this study. First, VSR1 functioned as a sorting receptor for beta-conglycinin by...
Topics
- Antigens, Plant
- Arabidopsis
- Arabidopsis Proteins
- Biological Transport
- Globulins
- Green Fluorescent Proteins
- Mutation
- Protein Sorting Signals
- Protein Transport
- Recombinant Fusion Proteins
- Seed Storage Proteins
- Seeds