Article
Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS.
The FEBS journal - 1 Feb 2007
Neuhof Torsten, Dieckmann Ralf, Druzhinina Irina S, Kubicek Christian P, Nakari-Setälä Tiina, Penttilä Merja, von Döhren Hans
Abstract excerpt
Intact-cell MS (ICMS) was applied for the direct detection of hydrophobins in various species and strains of Hypocrea/Trichoderma. In both mycelia and spores, dominating peaks were identified as hydrophobins by detecting mass shifts of 8 Da of reduced and unreduced forms, the analysis of knockout mutants, and comparison with protein databases. Strain-specific processing was observed in the case of Hypocrea...
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