Article
Tetraploid somatic hybrids of potato (Solanum tuberosum L.) obtained from diploid breeding lines.
Cellular & molecular biology letters - 1 Jan 2007
Przetakiewicz Jarosław, Nadolska-Orczyk Anna, Kuć Dominik, Orczyk Wacław
Abstract excerpt
Intraspecific somatic hybrids between 16 different diploid breeding lines of Solanum tuberosum L. were produced by PEG-induced fusion. Manually selected heterokaryons were cultured in a Millicells-CM using a post-fusion protoplast mixture. Plants were regenerated from calli derived from heterokaryons obtained from 10 out of 38 combinations of diploid lines. Of the tested putative somatic hybrids, 14.2% were...
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